Usage Of Microscope Slides

1. The smear method is a filmmaking method that evenly coats the material on the glass slide.

Smear materials include single-celled organisms, small algae, blood, bacterial culture fluid, loose tissues of animals and plants, seminal nests, anthers, etc.

When smearing, attention should be paid to: (1) The slide must be clean. (2) The slides should be flat. (3) The coating must be uniform. Apply the droplets to the right in the middle of the slide and apply well with a scalpel blade or toothpick. (4) The coating should be thin. Use another slide as a push sheet, gently push along the surface of the slide dripping with the coating solution (the angle between the two slides should be 30°-45°) from right to left, and coat a uniform thin layer. (5) Fixed. If fixation is required, it can be fixed with chemical fixatives or drying (bacteria). (6) Dyeing. Methylene blue for bacteria, Reye's staining for blood, and sometimes iodine wine. The staining solution should cover the entire surface. (7) Rinse. Blot dry or bake dry with absorbent paper. (8) Seal. Canadian gum seal for long-term storage.

2. The tablet pressing method is a preparation method in which the biological material is placed between the glass slide and the cover sheet, and a certain pressure is applied to disperse the tissue cells.

3. The loading method is a method of making slide specimens by sealing biological materials as a whole, and temporary or permanent mounting can be made by this method.

Loading materials include: microscopic organisms such as chlamydomonas algae, sponge, amoebas, nematodes; hydra, the leaf epidermis of plants; Insect wings, feet, mouthparts, human oral epithelial cells, etc.

Attention should be paid to the production of the loading method: (1) When holding the slide, it should be paid attention to flattening or placing it on the platform. When dripping, the amount of water should be appropriate to be just enough to be covered by the coverslip. (2) The material should be unfolded with a dissecting needle or forceps without overlapping and flattened on the same plane. (3) When placing the coverslip, slowly cover the water droplets from one side to prevent bubbles. (4) When staining, a drop of staining liquid is placed on one side of the coverslip and attracted from the other side with absorbent paper to evenly color the specimen under the coverslip. After coloring, use the same method, drop a drop of water, suck out the staining solution, and observe it under a microscope.

4. Slice is a slide specimen made from thin slices cut from living organisms.

Depending on the requirements, freehand sections can be performed with a blade, or tissue blocks can be embedded in paraffin or collodion or frozen at low temperatures and sliced with a microtome. Cut into 5~10 micron sheets for light microscope observation. Ultrathin sections cut with epoxy resin or methacrylic acid embedded tissue blocks, with a thickness of 20~50 nanometers, are specially designed for observation under electron microscopes. Sections used for general teaching, such as root tips and stems, are generally called paraffin sections.